ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (8): 1330-1335.doi: 10.11843/j.issn.0366-6964.2014.08.018

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Comparation of Codon Usage in Gene gp37 between Six T4-like Phages

LU Kai,HAN Shan-shan,ZHANG Can*,LIU Wen-hua,ZOU Ling,WEN Jian-xin,REN Hui-ying*   

  1. (College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China)
  • Received:2013-12-27 Online:2014-08-23 Published:2014-08-23

Abstract:

Polyvalent coliphage Bp7 was isolated and identified as T4-like phage,the codon usage in gene gp37 was analyzed by various bioinformatics softwares.The results were showed as follows:The patterns of codon usage in gene gp37 of phage Bp7 and other 5 T4-like phages were almost similar.In gene gp37 of phage Bp7,the codon bias was a little low,and gp37 seemed like using A or U ending codon;17 kinds of high-frequency codons were identified except AUG (Met) and UGG (Trp).The codon bias comparison of gp37 among phage Bp7 and other 5 phages showed that codon usage in Bp7 was most similar to JS98,a little far from T4 and T4T relatively.The codon usage patterns of gp37 may be influenced not only by selection pressure,but also by neutral mutation.The results of anlysis of gp37 codons will benefit to the modification of tail fiber protein of Bp7 and further broaden the host range of phage Bp7,and improve the level of gp37 expression as well.

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